AA 5564 then AA 2576 at Charlotte
Harrisburg → Charlotte → Chicago O'Hare
Timetable, Charlotte time
18:46AA 5564 from Harrisburg at the gate
18:40AA 2576 to Chicago O'Hare leaves the gate
23 h 54 apart on the timetable, overnight
AA 2576 leaves 23 h 54 after AA 5564 arrives on the timetable: more than half a day, so not a connection we count.