AA 5564 then AA 5222 at Charlotte
Harrisburg → Charlotte → Washington
Timetable, Charlotte time
18:46AA 5564 from Harrisburg at the gate
17:15AA 5222 to Washington leaves the gate
22 h 29 apart on the timetable, overnight
AA 5222 leaves 22 h 29 after AA 5564 arrives on the timetable: more than half a day, so not a connection we count.