AA 5564 then AA 5258 at Charlotte
Harrisburg → Charlotte → Lexington
Timetable, Charlotte time
18:46AA 5564 from Harrisburg at the gate
17:16AA 5258 to Lexington leaves the gate
22 h 30 apart on the timetable, overnight
AA 5258 leaves 22 h 30 after AA 5564 arrives on the timetable: more than half a day, so not a connection we count.