AA 5564 then AA 5543 at Charlotte
Harrisburg → Charlotte → Pittsburgh
Timetable, Charlotte time
18:46AA 5564 from Harrisburg at the gate
08:35AA 5543 to Pittsburgh leaves the gate
13 h 49 apart on the timetable, overnight
AA 5543 leaves 13 h 49 after AA 5564 arrives on the timetable: more than half a day, so not a connection we count.