AA 5567 then AA 5258 at Charlotte
Asheville → Charlotte → Lexington
Timetable, Charlotte time
17:20AA 5567 from Asheville at the gate
17:16AA 5258 to Lexington leaves the gate
23 h 56 apart on the timetable, overnight
AA 5258 leaves 23 h 56 after AA 5567 arrives on the timetable: more than half a day, so not a connection we count.