AA 5572 then AA 1548 at Charlotte
Lexington → Charlotte → Washington National
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
14:29AA 1548 to Washington National leaves the gate
23 h 27 apart on the timetable, overnight
AA 1548 leaves 23 h 27 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.