AA 5572 then AA 2255 at Charlotte
Lexington → Charlotte → Pittsburgh
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
08:55AA 2255 to Pittsburgh leaves the gate
17 h 53 apart on the timetable, overnight
AA 2255 leaves 17 h 53 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.