AA 5572 then AA 5055 at Charlotte
Lexington → Charlotte → Louisville
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
13:15AA 5055 to Louisville leaves the gate
22 h 13 apart on the timetable, overnight
AA 5055 leaves 22 h 13 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.