AA 5572 then AA 5075 at Charlotte
Lexington → Charlotte → Roanoke
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
14:31AA 5075 to Roanoke leaves the gate
23 h 29 apart on the timetable, overnight
AA 5075 leaves 23 h 29 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.