AA 5572 then AA 5941 at Charlotte
Lexington → Charlotte → Tri-Cities
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
14:35AA 5941 to Tri-Cities leaves the gate
23 h 33 apart on the timetable, overnight
AA 5941 leaves 23 h 33 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.