AA 5572 then AA 5986 at Charlotte
Lexington → Charlotte → Birmingham (Alabama)
Timetable, Charlotte time
15:02AA 5572 from Lexington at the gate
13:40AA 5986 to Birmingham (Alabama) leaves the gate
22 h 38 apart on the timetable, overnight
AA 5986 leaves 22 h 38 after AA 5572 arrives on the timetable: more than half a day, so not a connection we count.