AA 5574 then AA 5655 at Charlotte
Lexington → Charlotte → Manchester
Timetable, Charlotte time
19:39AA 5574 from Lexington at the gate
19:29AA 5655 to Manchester leaves the gate
23 h 50 apart on the timetable, overnight
AA 5655 leaves 23 h 50 after AA 5574 arrives on the timetable: more than half a day, so not a connection we count.