AA 5585 then AA 2725 at Charlotte
Louisville → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
19:21AA 5585 from Louisville at the gate
15:00AA 2725 to Charleston (South Carolina) leaves the gate
19 h 39 apart on the timetable, overnight
AA 2725 leaves 19 h 39 after AA 5585 arrives on the timetable: more than half a day, so not a connection we count.