AA 5585 then AA 3306 at Charlotte
Louisville → Charlotte → Cincinnati
Timetable, Charlotte time
20:41AA 5585 from Louisville at the gate
20:30AA 3306 to Cincinnati leaves the gate
23 h 49 apart on the timetable, overnight
AA 3306 leaves 23 h 49 after AA 5585 arrives on the timetable: more than half a day, so not a connection we count.