AA 5585 then AA 5164 at Charlotte
Louisville → Charlotte → Knoxville
Timetable, Charlotte time
19:21AA 5585 from Louisville at the gate
18:21AA 5164 to Knoxville leaves the gate
23 h apart on the timetable, overnight
AA 5164 leaves 23 h after AA 5585 arrives on the timetable: more than half a day, so not a connection we count.