AA 5585 then AA 5541 at Charlotte
Louisville → Charlotte → Cincinnati
Timetable, Charlotte time
20:41AA 5585 from Louisville at the gate
20:25AA 5541 to Cincinnati leaves the gate
23 h 44 apart on the timetable, overnight
AA 5541 leaves 23 h 44 after AA 5585 arrives on the timetable: more than half a day, so not a connection we count.