AA 5586 then AA 6110 at Charlotte
Sarasota → Charlotte → Knoxville
Timetable, Charlotte time
16:57AA 5586 from Sarasota at the gate
14:50AA 6110 to Knoxville leaves the gate
21 h 53 apart on the timetable, overnight
AA 6110 leaves 21 h 53 after AA 5586 arrives on the timetable: more than half a day, so not a connection we count.