AA 5601 then AA 3265 at Charlotte
Nashville → Charlotte → Madison
Timetable, Charlotte time
21:50AA 5601 from Nashville at the gate
21:44AA 3265 to Madison leaves the gate
23 h 54 apart on the timetable, overnight
AA 3265 leaves 23 h 54 after AA 5601 arrives on the timetable: more than half a day, so not a connection we count.