AA 5601 then AA 5266 at Charlotte
Nashville → Charlotte → Lexington
Timetable, Charlotte time
21:50AA 5601 from Nashville at the gate
21:45AA 5266 to Lexington leaves the gate
23 h 55 apart on the timetable, overnight
AA 5266 leaves 23 h 55 after AA 5601 arrives on the timetable: more than half a day, so not a connection we count.