AA 5601 then AA 5533 at Charlotte
Nashville → Charlotte → Gulfport
Timetable, Charlotte time
21:50AA 5601 from Nashville at the gate
21:35AA 5533 to Gulfport leaves the gate
23 h 45 apart on the timetable, overnight
AA 5533 leaves 23 h 45 after AA 5601 arrives on the timetable: more than half a day, so not a connection we count.