AA 5639 then AA 5258 at Charlotte
Cincinnati → Charlotte → Lexington
Timetable, Charlotte time
17:25AA 5639 from Cincinnati at the gate
17:16AA 5258 to Lexington leaves the gate
23 h 51 apart on the timetable, overnight
AA 5258 leaves 23 h 51 after AA 5639 arrives on the timetable: more than half a day, so not a connection we count.