AA 580 then AA 2532 at Charlotte
Seattle → Charlotte → Rochester
Timetable, Charlotte time
20:43AA 580 from Seattle at the gate
19:51AA 2532 to Rochester leaves the gate
23 h 08 apart on the timetable, overnight
AA 2532 leaves 23 h 08 after AA 580 arrives on the timetable: more than half a day, so not a connection we count.