AA 580 then AA 5318 at Charlotte
Seattle → Charlotte → Lexington
Timetable, Charlotte time
20:43AA 580 from Seattle at the gate
19:28AA 5318 to Lexington leaves the gate
22 h 45 apart on the timetable, overnight
AA 5318 leaves 22 h 45 after AA 580 arrives on the timetable: more than half a day, so not a connection we count.