AA 580 then AA 5376 at Charlotte
Seattle → Charlotte → Columbia Metropolitan
Timetable, Charlotte time
20:43AA 580 from Seattle at the gate
20:15AA 5376 to Columbia Metropolitan leaves the gate
23 h 32 apart on the timetable, overnight
AA 5376 leaves 23 h 32 after AA 580 arrives on the timetable: more than half a day, so not a connection we count.