AA 5807 then AA 5543 at Charlotte
Wilmington → Charlotte → Pittsburgh
Timetable, Charlotte time
11:34AA 5807 from Wilmington at the gate
08:35AA 5543 to Pittsburgh leaves the gate
21 h 01 apart on the timetable, overnight
AA 5543 leaves 21 h 01 after AA 5807 arrives on the timetable: more than half a day, so not a connection we count.