AA 5809 then AA 2255 at Charlotte
Harrisburg → Charlotte → Pittsburgh
Timetable, Charlotte time
09:15AA 5809 from Harrisburg at the gate
08:55AA 2255 to Pittsburgh leaves the gate
23 h 40 apart on the timetable, overnight
AA 2255 leaves 23 h 40 after AA 5809 arrives on the timetable: more than half a day, so not a connection we count.