AA 5809 then AA 5377 at Charlotte
Harrisburg → Charlotte → Rochester
Timetable, Charlotte time
09:15AA 5809 from Harrisburg at the gate
09:08AA 5377 to Rochester leaves the gate
23 h 53 apart on the timetable, overnight
AA 5377 leaves 23 h 53 after AA 5809 arrives on the timetable: more than half a day, so not a connection we count.