AA 5825 then AA 5222 at Charlotte
Knoxville → Charlotte → Washington
Timetable, Charlotte time
17:36AA 5825 from Knoxville at the gate
17:15AA 5222 to Washington leaves the gate
23 h 39 apart on the timetable, overnight
AA 5222 leaves 23 h 39 after AA 5825 arrives on the timetable: more than half a day, so not a connection we count.