AA 5825 then AA 5258 at Charlotte
Knoxville → Charlotte → Lexington
Timetable, Charlotte time
17:36AA 5825 from Knoxville at the gate
17:16AA 5258 to Lexington leaves the gate
23 h 40 apart on the timetable, overnight
AA 5258 leaves 23 h 40 after AA 5825 arrives on the timetable: more than half a day, so not a connection we count.