AA 5831 then AA 2189 at Charlotte
Tri-Cities → Charlotte → Madison
Timetable, Charlotte time
16:41AA 5831 from Tri-Cities at the gate
16:40AA 2189 to Madison leaves the gate
23 h 59 apart on the timetable, overnight
AA 2189 leaves 23 h 59 after AA 5831 arrives on the timetable: more than half a day, so not a connection we count.