AA 5831 then AA 3939 at Charlotte
Tri-Cities → Charlotte → Key West
Timetable, Charlotte time
16:41AA 5831 from Tri-Cities at the gate
15:35AA 3939 to Key West leaves the gate
22 h 54 apart on the timetable, overnight
AA 3939 leaves 22 h 54 after AA 5831 arrives on the timetable: more than half a day, so not a connection we count.