AA 5831 then AA 5677 at Charlotte
Tri-Cities → Charlotte → Cincinnati
Timetable, Charlotte time
16:41AA 5831 from Tri-Cities at the gate
16:02AA 5677 to Cincinnati leaves the gate
23 h 21 apart on the timetable, overnight
AA 5677 leaves 23 h 21 after AA 5831 arrives on the timetable: more than half a day, so not a connection we count.