AA 5831 then AA 6131 at Charlotte
Tri-Cities → Charlotte → Jacksonville (North Carolina)
Timetable, Charlotte time
16:41AA 5831 from Tri-Cities at the gate
15:42AA 6131 to Jacksonville (North Carolina) leaves the gate
23 h 01 apart on the timetable, overnight
AA 6131 leaves 23 h 01 after AA 5831 arrives on the timetable: more than half a day, so not a connection we count.