AA 5831 then AA 6445 at Charlotte
Tri-Cities → Charlotte → Knoxville
Timetable, Charlotte time
16:41AA 5831 from Tri-Cities at the gate
15:35AA 6445 to Knoxville leaves the gate
22 h 54 apart on the timetable, overnight
AA 6445 leaves 22 h 54 after AA 5831 arrives on the timetable: more than half a day, so not a connection we count.