AA 5839 then AA 3221 at Charlotte
Newport News → Charlotte → Tampa
Timetable, Charlotte time
07:37AA 5839 from Newport News at the gate
07:28AA 3221 to Tampa leaves the gate
23 h 51 apart on the timetable, overnight
AA 3221 leaves 23 h 51 after AA 5839 arrives on the timetable: more than half a day, so not a connection we count.