AA 5843 then AA 5412 at Charlotte
Roanoke → Charlotte → Daytona Beach
Timetable, Charlotte time
12:23AA 5843 from Roanoke at the gate
11:41AA 5412 to Daytona Beach leaves the gate
23 h 18 apart on the timetable, overnight
AA 5412 leaves 23 h 18 after AA 5843 arrives on the timetable: more than half a day, so not a connection we count.