AA 5924 then AA 2854 at Charlotte
Gainesville → Charlotte → Louisville
Timetable, Charlotte time
15:29AA 5924 from Gainesville at the gate
14:20AA 2854 to Louisville leaves the gate
22 h 51 apart on the timetable, overnight
AA 2854 leaves 22 h 51 after AA 5924 arrives on the timetable: more than half a day, so not a connection we count.