AA 5924 then AA 5543 at Charlotte
Gainesville → Charlotte → Pittsburgh
Timetable, Charlotte time
15:29AA 5924 from Gainesville at the gate
08:35AA 5543 to Pittsburgh leaves the gate
17 h 06 apart on the timetable, overnight
AA 5543 leaves 17 h 06 after AA 5924 arrives on the timetable: more than half a day, so not a connection we count.