AA 5941 then AA 2885 at Charlotte
Tri-Cities → Charlotte → Jacksonville
Timetable, Charlotte time
17:23AA 5941 from Tri-Cities at the gate
17:20AA 2885 to Jacksonville leaves the gate
23 h 57 apart on the timetable, overnight
AA 2885 leaves 23 h 57 after AA 5941 arrives on the timetable: more than half a day, so not a connection we count.