AA 5941 then AA 5222 at Charlotte
Tri-Cities → Charlotte → Washington
Timetable, Charlotte time
17:23AA 5941 from Tri-Cities at the gate
17:15AA 5222 to Washington leaves the gate
23 h 52 apart on the timetable, overnight
AA 5222 leaves 23 h 52 after AA 5941 arrives on the timetable: more than half a day, so not a connection we count.