AA 5972 then AA 5377 at Charlotte
Mobile → Charlotte → Rochester
Timetable, Charlotte time
10:19AA 5972 from Mobile at the gate
09:08AA 5377 to Rochester leaves the gate
22 h 49 apart on the timetable, overnight
AA 5377 leaves 22 h 49 after AA 5972 arrives on the timetable: more than half a day, so not a connection we count.