AA 5984 then AA 1393 at Charlotte
Florence → Charlotte → Des Moines
Timetable, Charlotte time
16:51AA 5984 from Florence at the gate
16:15AA 1393 to Des Moines leaves the gate
23 h 24 apart on the timetable, overnight
AA 1393 leaves 23 h 24 after AA 5984 arrives on the timetable: more than half a day, so not a connection we count.