AA 5987 then AA 5543 at Charlotte
Gainesville → Charlotte → Pittsburgh
Timetable, Charlotte time
16:30AA 5987 from Gainesville at the gate
08:35AA 5543 to Pittsburgh leaves the gate
16 h 05 apart on the timetable, overnight
AA 5543 leaves 16 h 05 after AA 5987 arrives on the timetable: more than half a day, so not a connection we count.