AA 5989 then AA 2562 at Charlotte
Gainesville → Charlotte → St Louis
Timetable, Charlotte time
21:24AA 5989 from Gainesville at the gate
20:52AA 2562 to St Louis leaves the gate
23 h 28 apart on the timetable, overnight
AA 2562 leaves 23 h 28 after AA 5989 arrives on the timetable: more than half a day, so not a connection we count.