AA 5990 then AA 5262 at Charlotte
Charlottesville → Charlotte → Lexington
Timetable, Charlotte time
08:02AA 5990 from Charlottesville at the gate
07:17AA 5262 to Lexington leaves the gate
23 h 15 apart on the timetable, overnight
AA 5262 leaves 23 h 15 after AA 5990 arrives on the timetable: more than half a day, so not a connection we count.