AA 6075 then AA 5377 at Charlotte
Roanoke → Charlotte → Rochester
Timetable, Charlotte time
09:22AA 6075 from Roanoke at the gate
09:08AA 5377 to Rochester leaves the gate
23 h 46 apart on the timetable, overnight
AA 5377 leaves 23 h 46 after AA 6075 arrives on the timetable: more than half a day, so not a connection we count.