AA 749 then AA 5543 at Charlotte
Madrid → Charlotte → Pittsburgh
Timetable, Charlotte time
16:25AA 749 from Madrid at the gate
08:35AA 5543 to Pittsburgh leaves the gate
16 h 10 apart on the timetable, overnight
AA 5543 leaves 16 h 10 after AA 749 arrives on the timetable: more than half a day, so not a connection we count.