AA 878 then AA 5318 at Charlotte
Orlando → Charlotte → Lexington
Timetable, Charlotte time
20:01AA 878 from Orlando at the gate
19:28AA 5318 to Lexington leaves the gate
23 h 27 apart on the timetable, overnight
AA 5318 leaves 23 h 27 after AA 878 arrives on the timetable: more than half a day, so not a connection we count.