UA 2301 then AA 5126 at Charlotte
Newark → Charlotte → Louisville
Timetable, Charlotte time
22:13UA 2301 from Newark at the gate
22:05AA 5126 to Louisville leaves the gate
23 h 52 apart on the timetable, overnight
AA 5126 leaves 23 h 52 after UA 2301 arrives on the timetable: more than half a day, so not a connection we count.