UA 2301 then AA 5533 at Charlotte
Newark → Charlotte → Gulfport
Timetable, Charlotte time
21:41UA 2301 from Newark at the gate
21:35AA 5533 to Gulfport leaves the gate
23 h 54 apart on the timetable, overnight
AA 5533 leaves 23 h 54 after UA 2301 arrives on the timetable: more than half a day, so not a connection we count.